Selection of oligonucleotide probes for detection of mRNA isoforms.
نویسندگان
چکیده
منابع مشابه
Selection of Oligonucleotide Probes for Protein Coding Sequences
MOTIVATION Large arrays of oligonucleotide probes have become popular tools for analyzing RNA expression. However to date most oligo collections contain poorly validated sequences or are biased toward untranslated regions (UTRs). Here we present a strategy for picking oligos for microarrays that focus on a design universe consisting exclusively of protein coding regions. We describe the constra...
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15 صفحه اولSelective Oligonucleotide and MRNA Pull-Down with Shielded Covalent Probes
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Demonstration of mRNA using digoxigenin labelled oligonucleotide probes for in situ hybridisation in formamide free conditions.
The value of formamide for use in in situ hybridisation (ISH) for the detection of mRNA, using either single or multi probe cocktails of digoxigenin labelled oligoprobes, was investigated. Three peptides with cell specific localisation in three separate tissues--calcitonin in the thyroid, epidermal growth factor (EGF) in the submaxillary gland, and insulin in the pancreas were studied. In each ...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1993
ISSN: 0022-1554,1551-5044
DOI: 10.1177/41.12.8245420